Although goat meat supply has increased consistently over the past few decades, it still cannot meet the demand in many countries Devendra,
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Abstract There are many PCR-based methods for animal species identification; however, their detection numbers are limited or could not identify unknown species.
We set out to solve this problem by developing a universal primer PCR assay for simultaneous identification of eight animal species, including goat, sheep, deer, buffalo, cattle, yak, pig, and camel. In this assay, the variable lengths of mitochondrial DNA were amplified using a pair of universal primers.
PCR amplifications yielded bp, bp, bp, bp, bp, bp, bp, and bp length fragments for goat, sheep, deer, buffalo, cattle, yak, pig, and camel, respectively.
This primer pair had no cross-reaction with other common domestic animals and fish.
The limit of detection varied from 0. Each PCR product could be further digested into fragments with variable sizes and qualitative analysis by SspI restriction enzyme.
Compared with the previous published related methods, this approach is simple, with high throughput, fast processing rates, and more cost-effective for routine identification of meat in foodstuffs. Introduction The consumption of meat and meat products is increasing each year in the world.
On the other hand, food authenticity issues in the form of adulteration and improper description have existed for as long as food has been offered for sale. However, meat adulteration affects food safety, quality, and many other respects, becoming a public focus recently.
Since the s, many immunological and molecular methods for species identification in food products have been developed [ 1 — 8 ].
Concomitant with advances in large-scale integrated molecular technology, DNA analysis methods have been in rapid development. Many assays based on DNA analysis, especially PCR-based methods, have been developed for species identification in foodstuffs.
Though each of these methods has its own advantages and disadvantages, the number of species identified in a single PCR was limited to [ 9 ]. Compared with other PCR methods, the combination of universal primer PCR and restriction fragment length polymorphism RFLP assays has advantages of increased simplicity, specificity, and sensitivity and has been used in many meat identification practices [ 10 — 17 ].
On the other hand, a few modified PCR assays combining universal primer with specific primer or RFLP had been developed [ 1819 ]; these methods increased efficiency and versatility. With the development of Western China, more and more local meat products of mutton, beef, yak, deer, and camel have been sold to all parts of the country.
There have been a few analytical methods for the identification of yak [ 2021 ], deer [ 17 ], buffalo [ 2223 ], and camel [ 24 ] meat in food products, but to our knowledge, there is no one method for simultaneously identifying two and more animal species, for goat, sheep, buffalo, cattle, yak, deer, and camel.
Furthermore, pork is popular with Chinese people, but Muslim populations are widely distributed in China; all the meat products from pork source are forbidden in halal foods.
In this regard, precise identification of meat origin has become a vital element in food quality control procedures. Thus, the development of reliable, simple, and sensitive analytical methods for evaluating meat authenticity is of critical importance.Goat meat, often called chevon when from adults and cabrito or just kid when from young animals, is the meat of the domestic goat (Capra aegagrus hircus).
While "goat" is usually the name for the meat found in common parlance, producers and marketers may prefer to use the French-derived word chevon (from chÃ¨vre), since market research in the. May 29, · Goat meat, also known as chevon, is a lean source of high quality protein that is consumed in many parts of the world to differing degrees.
Goats are adaptable animals that can be raised in a variety of climates, thereby increasing their popularity as meat animals.
The supply of chevon, goat meat is seasonal. Make your own homemade breakfast sausage patties using good quality ingredients combined with fresh herbs and spices. - leave out brown sugar for homemade goat prosciutto capra dry cured mutton whole leg ham goat . page 1 1 effect of supplementing bahiagrass hay with warm season legume hays on feed intake, digestibility, nitrogen retention, body weight gain and parasite burden of goat kids by joseph chakana hamie a thesis submitted to the gradu ate school of the university of florida in partial fulfillment of the requirements for the degree of master of science university of florida Arslan et al.
() discriminated meats from certain domestic animals as goat, cattle, camel, sheep, pork and rabbit as well as meat from wild swine, donkey, dog and cat by PCR-RAPD using 10 base primer on both individual and mixed meat samples.
Indian and Nubian derived goat breeds are dual-purpose meat and milk producers. All these are herbivorous mammals.
the domestic goat evolved principally from capra aegagrus. the Spanish goat has outward-upward curving horns with a sharp posterior keel.
the US ''Spanish'' goats and most of the small or nondescript goats. Fleeces also.